Immunocytochemistry/Immunofluorescence: E-Selectin/CD62E Antibody (208) [NBP2-89432] - Staining of CD62E in TNF-a stimulated HUVEC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human ABHD14B monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.